DNA

Part:BBa_K300996:Design

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-02)

SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA scar designed considering AvrII restriction site and SpeI restriction site in RFC10 BioBrick Suffix.

Source

Datasheets of the commercially available SpeI and AvrII restriction enzymes and the RFC10 BioBrick Suffix sequence.

References

http://www.roche-applied-science.com/pack-insert/1008943a.pdf

https://parts.igem.org/Help:BioBrick_Prefix_and_Suffix